Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics

Evolving Accelerated Amidation by SpyTag/SpyCatcher to Analyze Membrane Dynamics

SpyTag is a peptide that forms a spontaneous amide bond with its protein partner SpyCatcher. This protein superglue is a broadly useful tool for molecular assembly, locking together biological building blocks efficiently and irreversibly in diverse architectures. We initially developed SpyTag and SpyCatcher by rational design, through splitting a domain from a Gram-positive bacterial adhesin. I...

Using Critics to Analyze Evolving Architectures Jason

Software architectures evolve as the result of numerous, interrelated design decisions. At any point in an architecture's evolution, current decisions can critically affect alternatives at later stages, and each decision has the potential of requiring previous decisions to be reconsidered. Analysis techniques that provide feedback only after " complete " sequences of design decisions have been ...

Empowering End-users to Collaboratively Analyze Evolving Complex Linked Data

Approaches to Analyze Protein-Protein Interactions of Membrane Proteins

It can be estimated that most membrane proteins function in complexes (Fig. 1) (Daley 2008). Protein-protein interactions (PPIs) within these complexes can either be direct (primary interaction) or indirect (secondary interaction). Direct interactions occur either by homooligomerisation as determined for bacterial two-component systems (Gao and Stock 2009) (Fig. 1A) or by hetero-oligomerisation...

Using System Dynamics to Analyze Customer Experience Design